mrna differential display การใช้

• In addition , more than 30 differential expressed sequence tags ( est ) have been isolated by mrna differential display
• Discussion in the process of development , differentiation and metabolism , the differential expression of genes plays a pivotal role . mrna differential display , established by liang and pardee in 1992 , is among the most sensitive methods for the isolation of differentially regulated mrna
• In addition , the well retained stability and integrity of cell membrane of boea leaves might also be an important mechanism which make them resurrect well . by using mrna differential display , 5 desiccation sensitive cdnas , 52 desiccation - induced cdnas , 21 up - regulated cdnas , 14 down - regulated cdnas and 16 phosphate induced cdnas were obtained . the cloning , sequencing , homological blasting and northern blotting results of 5 desiccation - induced cdnas and 3 phosphate induced cdnas implied that signal transduction induced by desiccation , regulatory gene cascades and functional genes such as g protein , protein kinase , vp3 - and mad3 - like genes might be involved in dehydration in the resurrection plant boea hygrometrica
• Were used to investigate the dehydration protection in the physiological and chemical levels represented by the changes of photosynthesis , sucrose , antioxidants and electrolyte leakage during desiccation and subsequent rehydration , and molecular mechanisms of dehydration tolerance by means of mrna differential display based on the studies of deng ' s